Identification of mutations in SARS-CoV-2 PCR primer regionsArticle Published on 2022-11-042022-11-15 Journal: Scientific Reports [Category] SARS, 변종, 치료기술, 치료제, [키워드] Accuracy Analysis anticipated approach dataset diagnostic Efficiency genomic variation gold standard identification Mutation Neutral omicron overlapping PCR PCR efficiency primer primer set primer sets Region RT-PCR Sample SARS-CoV-2 SARS-CoV-2 PCR SARS-CoV-2 viral strain susceptible target region the SARS-CoV-2 genome variant Variation [DOI] 10.1038/s41598-022-21953-3 PMC 바로가기
Development of a Loop-Mediated Isothermal Amplification Method for Rapid and Visual Detection of Monkeypox VirusArticle Published on 2022-10-262022-11-16 Journal: Microbiology Spectrum [Category] 치료기술, [키워드] amplification caused clinical sample clinical samples COVID-19 pandemic detection Detection limit development Diagnosis early stage evaluated hospital Infection isothermal isothermal amplification Loop-mediated isothermal amplification Monkeypox Monkeypox virus orthopoxvirus outbreak pathogen pathogenic virus Pathogens PCR primer set Primers public health threat Rapid rapid detection sensitivity sequence specificity Symptom temperature virus Visual visual detection. [DOI] 10.1128/spectrum.02714-22 PMC 바로가기
Wastewater-based epidemiology in countries with poor wastewater treatment – Epidemiological indicator function of SARS-CoV-2 RNA in surface waters폐수 처리가 열악한 국가의 폐수 기반 역학 - 지표수에서 SARS-CoV-2 RNA의 역학 지표 기능Article Published on 2022-10-152022-09-11 Journal: The Science of the total environment [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, 치료법, 치료제, [키워드] adaptation adoption affected alternative analysed applied approach Bacteria Belgrade collected complex complex systems Concentration country COVID-19 Danube effort epidemiological epidemiological purposes Epidemiology Factor Factors Faecal pollution Genes Genetic human populations identify indicated methodology N1 and N2 nucleocapsid outbreak parameter physicochemical primer set primer sets Protein qualitative data quantification quantified Quantitative recipient river RNA RT-qPCR SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 surveillance Sava Serbia surface Surface waters Surveillance Treatment Untreated wastewaters. Volume water [DOI] 10.1016/j.scitotenv.2022.156964 PMC 바로가기 [Article Type] Article
RT-LAMP assay combining multi-fluorescent probes for SARS-CoV-2 RNA detection and variant differentiationSARS-CoV-2 RNA 검출 및 변이체 분화를 위한 다중 형광 프로브를 결합한 RT-LAMP 분석Article Published on 2022-10-012022-09-11 Journal: Talanta [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] addition applied can be used clinical setting clinical settings detect Fluorescent probes Genes isothermal amplification limit of detection Loop-mediated isothermal amplification molecular mutant Mutation N gene observation ORF1 ORF1ab P681H P681R positive result primer set probe quantification regions reverse transcription Reverse transcription loop-mediated isothermal amplification (RT-LAMP) RT-LAMP S gene SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 RNA detection SARS-CoV-2 viral RNA sensitivity simple specificity Spread Transcription type strain variant Variant differentiation. viral RNA detection virus wild type wild type strain wild-type [DOI] 10.1016/j.talanta.2022.123644 PMC 바로가기 [Article Type] Article
Detection of SARS-CoV-2 in different human biofluids using the loop-mediated isothermal amplification assay: A prospective diagnostic study in Fortaleza, Brazil루프 매개 등온 증폭 분석을 사용하여 다양한 인간 생체 유체에서 SARS-CoV-2 검출: 브라질 Fortaleza의 전향적 진단 연구Article Published on 2022-09-012022-09-11 Journal: Journal of medical virology [Category] COVID19(2023년), SARS, 치료기술, [키워드] 95% CI 95% confidence interval Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 adopted biofluid Brazil caused Chain Reaction collected coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 detect detection Diagnosis diagnostic evaluated gold gold standard human gene isothermal amplification LAMP Loop-mediated isothermal amplification management Molecular diagnosis Molecular diagnosis. Nasopharyngeal swab nasopharyngeal swabs ORF1 ORF1ab oropharyngeal swabs pandemic Patient polymerase chain polymerase chain reaction primer primer set Primers processed Quantitative quantitative polymerase chain reaction RT-LAMP RT-qPCR SARS-CoV-2 Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus specificity [DOI] 10.1002/jmv.27842 PMC 바로가기 [Article Type] Article
Identification of conserved regions from 230,163 SARS-CoV-2 genomes and their use in diagnostic PCR primer design230,163 SARS-CoV-2 게놈에서 보존 영역 식별 및 진단 PCR 프라이머 디자인에서의 사용Article Published on 2022-08-012022-09-11 Journal: Genes & genomics [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] addition Alpha amplification approach Beta bioinformatics can be used candidate Candidates characteristic conserved Coverage dataset Delta Diagnosis diagnostic Epsilon Eta evaluated exhibited False negative full-length Gamma genome sequence genomes genomic sequence GISAID GISAID EpiCoV database identification identify in silico Intensive Iota Multiple Multiple sequence alignment multiple sequence alignments NCBI nine NSP2 orf3a gene Outbreaks pandemic PCR PCR primer primer primer set primer sets Primers qPCR RdRP Region removal retrieved RNA sample RNA samples RT-qPCR S gene SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 genomes SARS-CoV-2 variant SARS-CoV-2. sequence Sequence alignment target viral genome viral sequences was reduced Zeta [DOI] 10.1007/s13258-022-01264-7 PMC 바로가기 [Article Type] Article
Improving RT-LAMP detection of SARS-CoV-2 RNA through primer set selection and combination프라이머 세트 선택 및 조합을 통한 SARS-CoV-2 RNA의 RT-LAMP 검출 개선Article Published on 2022-04-012022-09-11 Journal: PLoS ONE [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] adoption approach assays breadth Combination conditions Detection sensitivity Diagnostic method Effect evaluate expand identify Improving isothermal amplification knowledge LAMP Loop-mediated isothermal amplification molecular nucleic acid nucleic acid testing observé primer primer set primer sets prominence RT-LAMP RT-qPCR SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 RNA sensitivity Surveillance targets Transcription utility while [DOI] 10.1371/journal.pone.0254324 PMC 바로가기 [Article Type] Article
Profiling RT-LAMP tolerance of sequence variation for SARS-CoV-2 RNA detectionSARS-CoV-2 RNA 검출을 위한 서열 변이의 RT-LAMP 내성 프로파일링Article Published on 2022-03-242022-09-11 Journal: PLoS ONE [Category] SARS, 변종, 치료기술, 치료제, [키워드] adopted affecting amplification amplify bases clinical laboratory creating Critical deletions Detection sensitivity diagnostic diagnostic test diagnostic tests dramatic increase Effect highlight identify knowledge Laboratory LAMP molecular molecular diagnostic tests Mutation no effect nucleic acid nucleic acid amplification Point mutation primer primer set primer sets Profiling qPCR assays Reactions reduced regions RNA sequence robust RT-LAMP RT-qPCR RT-qPCR assays SARS-CoV-2 SARS-CoV-2 pandemic SARS-CoV-2 RNA detection SARS-CoV-2 sequence SARS-CoV-2 variant SARS-CoV-2 variants sensitivity sequence variation significantly Spread These data Tolerance utility variant Viral RNA viral RNA detection virus [DOI] 10.1371/journal.pone.0259610 PMC 바로가기 [Article Type] Article
High-Integrity Sequencing of Spike Gene for SARS-CoV-2 Variant DeterminationSARS-CoV-2 변이체 결정을 위한 스파이크 유전자의 높은 무결성 시퀀싱Article Published on 2022-03-172022-09-11 Journal: International Journal of Molecular Sciences [Category] SARS, 변종, 유전자 메커니즘, 치료기술, [키워드] amplicons approach ARTIC Network consensus sequence Coverage datasets demonstrated detect detection rate Determination determine Disadvantages evaluated Express FASTQ in silico mutations Nanopore nanopore sequencing overcome Oxford PCR PCR primer PCR primers primer primer set primer sets Primers protocol provided Region robustness SARS-CoV-2 SARS-CoV-2 genome sequence sequenced sample sequenced samples Sequencing spike spike gene Spike protein the SARS-CoV-2 genome variant Variant. variants [DOI] 10.3390/ijms23063257 PMC 바로가기 [Article Type] Article
Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCRTaqman 기반 및 SYBR 녹색 기반 RT-QPCR에 의한 SARS-COV-2 프라이머 세트의 분석 감도 및 효율 비교Methods and Protocols Published on 2022-02-262022-09-01 Journal: Applied Microbiology and Biotechnology [Category] COVID19(2023년), Fulltext, MERS, SARS, 변종, 진단, 치료기술, [키워드] alternative method analytical sensitivity approach best Combination comparable coronavirus disease Coronavirus disease 2019 country COVID-19 Delta detect Diagnosis diagnostics Efficiency Eta evaluated gold gold standard Health Organization Lambda molecular omicron ORF1 ORF1ab pandemic Patient Prevent primer set primer sets public health RT-qPCR SARS-CoV-2 SARS-CoV-2 detection sensitivity specificity supplementary material SYBR Green TaqMan TaqMan probe Vaccine variant variants WHO World Health Organization [DOI] 10.1007/s00253-022-11822-4 PMC 바로가기 [Article Type] Methods and Protocols